human total il 18  (R&D Systems)

Journal: bioRxiv

Article Title: Anti-oxidant and anti-inflammatory Effects of Aerosolised microalgal-derived extracellular vesicles in Bronchial Epithelial–Macrophage Co-cultures at the Air-Liquid Interface

doi: 10.64898/2026.03.19.712886

Figure Lengend Snippet: Cytokine secretion profile of CALU-3/dTHP-1 coculture following exposure to nebulized LPS. ( A ) IL-6, ( B ) IL-8, ( C ) IL-1β, ( D ) IL-18, (E) TNF-α, and ( F ) IL-10 were quantified after exposure to 125 and 250 µg/mL nebulized LPS solution for 24 hours. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined using one-way ANOVA (*p< 0.5, **p < 0.01).

Article Snippet: The levels of the pro-inflammatory cytokine IL-8 and IL-18 were measured using the Human IL-8/CXCL8 DuoSet ELISA Kit (R&D Systems, DY208) and Human Total IL-18 DuoSet ELISA (R&D Systems, DY318) following the manufacturer’s recommendations.

Techniques:

Journal: bioRxiv

Article Title: Stiffness Drives Endothelial Senescence and Inflammation in Aging and Doxorubicin-Induced Vascular Dysfunction

doi: 10.64898/2026.01.30.702284

Figure Lengend Snippet: (A) Schematic representation of the cell treatment protocol. For the Doxorubicin-treated (Doxo) samples (blue), cells were seeded at day -3, and Doxo was added at day 0 for 24h. For the control samples (green), cells were seeded at day 0, and treated with DMSO for 24h at day 3. For both conditions, the senescent assays were performed at day 7 after treatment. (B) Proliferation assay at day 7 following 24h Doxo treatment across doses from 65–1000 nM. Ratios represent cell number at day 7 relative to day 0 (mean ± SD). (C) Left: representative bright-field images of the β -Galactosidase ( β -Gal) staining at day 7, scale bar 100 µ m. Right: Representative immunofluorescence images: γ -H2AX (magenta), nuclei (blue); scale bar 20 µ m. (D) β -Gal positivity at day 7 (mean ± SD). (E) DNA damage measured by γ -H2AX foci per nucleus (mean ± SEM). (F) ELISA quantification of IL-6 and IL-8 secretion at day 7 (mean ± SD). Statistical analysis: Mann-Whitney t-test; N = 2, n = 3. Significance: * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001, **** ≤ 0.0001.

Article Snippet: Human IL-6 and IL-8 DuoSet ELISA kits (R&D Systems) were used according to the manufacturer’s instructions.

Techniques: Control, Proliferation Assay, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: bioRxiv

Article Title: Stiffness Drives Endothelial Senescence and Inflammation in Aging and Doxorubicin-Induced Vascular Dysfunction

doi: 10.64898/2026.01.30.702284

Figure Lengend Snippet: (A) Schematic representation of the protocol followed to obtain replicative senescence (RS). (B) Growth rate assessed as the ratio of cell number at day 7 to day 0 (mean ± SD). (C) Left: representative bright-field images of the β -Galactosidase ( β -Gal) staining at day 7, scale bar 100 µ m. Right: Representative immunofluorescence images: γ -H2AX (magenta), nuclei (blue); scale bar 20 µ m. (D) β -Gal positivity at day 7 (mean ± SD). (E) DNA damage measured by γ -H2AX foci per nucleus (mean ± SEM). (F) ELISA quantification of secreted IL-6 and IL-8 in RS cells versus controls. Statistical analysis: Mann–Whitney t-test. N=2–3, n=3. Significance: * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001, **** ≤ 0.0001.

Article Snippet: Human IL-6 and IL-8 DuoSet ELISA kits (R&D Systems) were used according to the manufacturer’s instructions.

Techniques: Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: bioRxiv

Article Title: Stiffness Drives Endothelial Senescence and Inflammation in Aging and Doxorubicin-Induced Vascular Dysfunction

doi: 10.64898/2026.01.30.702284

Figure Lengend Snippet: A) IL-6 and IL-8 secretion levels in control, Doxotreated, and RS populations after 7 days, quantified by ELISA and normalized to cell number (mean ± SEM). Statistical analysis: two-way ANOVA with Tukey’s post hoc test (ELISA), N=3, n=3. Significance: * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001, **** ≤ 0.0001. B) Gene Ontology (GO) enrichment analysis displaying the top 10 Biological Process (BP) terms with highest statistical significance for Control vs Doxo and Control vs RS comparisons, respectively. For all GO term graphs, vertical axis shows GO terms and horizontal axis shows GeneRatio which corresponds to the genes of interest in the sample gene set divided over the total number of genes in the GO term. The size of each term dot represents the total count of genes identified in the sample gene set belonging to the term and the colour describes statistical significance according to the p-adjust legend. Recurring terms include chemotaxis, leukocyte migration, and extracellular matrix organization. Statistical analysis: DESeq2 (RNA-seq). N=3, n=3.

Article Snippet: Human IL-6 and IL-8 DuoSet ELISA kits (R&D Systems) were used according to the manufacturer’s instructions.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, Migration, RNA Sequencing